Data/hora: 15/05/2024 20:50:35

Biblioteca(s): | Embrapa Hortaliças.
Data corrente: | 31/03/1993
Data da última atualização: | 05/03/2024
Autoria: | KITAJIMA, E. W.; RESENDE, R. de O.; AVILA, A. C. de; GOLDBACH, R. W.; PETERS, D.
Afiliação: | ANTONIO CARLOS DE AVILA, CNPH.
Título: | Immuno-electron microscopical detection of tomato spotted wilt virus and its nucleocapsids in crude plant extracts.
Ano de publicação: | 1992
Fonte/Imprenta: | Journal of Virological Methods, v. 38, n. 3, p. 313-322, Aug./Sept. 1992.
DOI: | https://doi.org/10.1016/0166-0934(92)90076-P
Idioma: | Inglês
Conteúdo: | Tomato spotted wilt virus (TSWV) particles were identified in thin sections and in crude extracts of leaves from plants infected with different TSWV isolates, using gold labelled protein A and antibodies prepared against purified virus particles or against nucleocapsid preparations. In addition, both in thin sections and in dip preparations aggregates were detected using either goldlabelled antiserum. These aggregates were not detected using antisera against envelope proteins or against the non-structural protein NSs. They were the only detectably labelled material in preparations made from plants infected by morphologically defective isolates of TSWV, i.e. isolates that did not produce enveloped particles. The aggregates were discerned in dip preparations as cloudy amorphous structures having a cottonwool boll-like appearance. These ‘cotton bolls’ were interpreted as being the in vitro structures of the nucleocapsid aggregates occurring in plant cells infected by isolates maintained by mechanical inoculation and are the only structures in cells infected by morphologically defective isolates (Ie, 1982; Kitajima et al., 1992). The detection of the ‘cotton boll-like’ structures in crude extracts provides an adequate and rapid method to establish the presence of nucleocapsid aggregates in cells of infected plants, irrespective of whether they are infected by morphologically defective isolates or not.
Palavras-Chave: | Deteccao; Immunogold; Imunoflorescencia; Labelling; TSWV; Vira-Cabeca.
Thesaurus Nal: | detection; Tomato spotted wilt virus.
Categoria do assunto: | S Ciências Biológicas
Registro original: | Embrapa Hortaliças (CNPH)
Exemplares: | CNPH - (970-1) - UPC - AP - DD - 13261 -  - 13261 - DIS